Analysis of all three nucleotide changes is performed using PCR amplification followed by restriction enzyme digest. |
If the restriction enzyme activities were inhibited, the DNA fragment would not be cleaved and should be seen in its original size on the gel. |
Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. |
The restriction enzyme bound on DNA at the first site binds its second site to a remote DNA sequence and then dissociates from the first one. |
Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing. |
The restriction enzyme and its corresponding methylase constitute the restriction-modification system of a bacterial species. |