| The distribution of 2 plasmid DNA topoisomers was measured by Southern blot analysis with a radiolabeled 2 DNA probe. |
| Consequently, the conjugational activity can be low when most cells carry the plasmid but increase greatly in response to plasmid-free cells. |
| For each transformation, the plasmid inserts from 7 to 18 colonies were sequenced. |
| Thus we were able to clone the DNA sequence that flanked the transposon insertion using plasmid rescue. |
| Strains were transformed with a control centromeric plasmid or a centromeric plasmid carrying the wild-type GUK1 gene. |
| The same can also be expressed intracellularly when introduced through plasmid or viral vectors. |
