How to use staining in a sentence

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Sentence Examples For microscopic analysis of induced cell death and fungal development, Trypan Blue staining was performed on individual leaves. The staining was predominantly located in endothelial and perivascular cells within the tumors. Interestingly, our results also showed that the leukemic cells retained the perinuclear, large, blocklike granular staining in 8 of 14 cases. It strongly stained the perineurium but did not stain the nerve fibers within the perineurium, thus giving a ringlike staining pattern. Histochemical staining showed lignin and suberin to be absent in both untreated and oleocellosis-damaged tissue. Polymerase chain reaction products were examined by agarose gel electrophoresis and ethidium bromide staining. In addition, a strong staining was observed in maternal chalazal tissue shortly after fertilization, and persisted throughout seed development. No changes in the staining of chalazal cell walls were detected at the end of grain filling. Sometimes iron sulfides have weathered, staining the quartz an orange color, both on the surface and within the crystals themselves. Though the arrangement was similar, isolated islets showed reduction in the staining of all the three cell types. The next morning we masked off the baseboards, doors, and windows with plastic and proceeded with staining. Before staining, you may want to apply a special wood sealer to the flooring. To minimize staining and wear and tear, Carmichael chooses cottons with a tight weave and a pattern. No staining in the retina or any other parts of the body was detected on sections. Perl cells inspected at LDT 9 had one dense spot of staining near the basal body of the flagella and less-intensely stained particles elsewhere. At week 9, patchy staining was observed with a few clusters of cells within the basal cell layer. The integumental wounds were easily detected on the body of infested bee pupae by vital staining with Trypan blue. A thick half-pound, grilled to a perfect medium, it oozes juice, staining the semi-fresh kaiser until it's ketchup red. Upon staining, these round bacteria are visualized in clumps that resemble bunches of grapes. No staining was detected when the preimmune serum was used as a negative control. Show More Sentences There also will be worse staining of the teeth and some risk of tooth loss from caries because of the sugar in chewing tobacco. The scarlet juice slowly dripped onto her dress, staining it with the redness of blood. Preliminary findings suggest that rotenone or the humic and fulvic acid treatments can slightly reduce staining. Mordants most commonly used in tissue staining are salts of aluminum, chromium, iron, potassium, and tungsten. His shin bone was all twisted out at a peculiar angle, and there was blood staining the cloth of his pants. All eyes studied that had characteristics of amyloid accumulation by hematoxylin-eosin staining were also stained with Congo red. New and practical staining techniques on frozen tissues, such as modified trichrome and crystal violet stains, are welcome additions. Anthers were dissected under a binocular microscope and pollen was gently squashed in staining solution under a coverslip. If you plan to stain end the grain, apply a wood conditioner before staining so the end won't soak up too much stain. A petrographer could examine a sample of the stained concrete and tell you whether the aggregate is likely to be the source of staining. Masson specified a dye he called Ponceau de xylidine for a variant of his trichrome staining method. The presence of antinuclear antibodies was tested by indirect immunofluorescent antibody staining of fixed cells. There was a tear in his left trouser leg, just above the knee, blood was staining the fabric there. A monolayer of cells is then placed on the glass slide for staining and manual screening. The cherries were staining her lips an enticing crimson colour, and Cary longed to lean across the table to kiss the juice away. Figure 3 shows typical staining patterns for uninfected and infected HeLa cells, which were used as controls. Cells were therefore processed for immunofluorescence staining with anti-tubulin antibodies in order to visualize spindle morphology. Predominantly in the tangential walls, primary pit fields of high density are conspicuously labelled by aniline blue staining of callose. Fraction purities were assessed by a combination of Coomassie staining and immunoblotting of electrophoretically separated proteins. Paul Ehrlich improved on Koch's staining procedure, using aniline instead of ammonia and fuchsin instead of methylene blue. Show More Sentences It was within the lepromatous skin nodules using primitive staining techniques that he saw masses of rodshaped bodies. He kicked the back flaps of his coat, a bit of slush staining the underside, but he didn't care, he needed to hurry. A mild acid is often added to amidol formulae to prevent oxidation, which can cause staining of the print. Immunohistochemically, the cells with ganglionic features showed focal perikaryal staining for synaptophysin and chromogranin. The letters were carved in a cramped scrawl, moonlight etching the crevices and staining the shadows silver. No staining was observed in animals mounted in levamisole but not irradiated, indicating that all expression of the transgene was laser induced. Slides were prepared using the same enzyme treatment employed for fluorochromes, except that they were not aged before staining. She was crying, shaking violently, her usually perfect makeup smudged, mascara staining her cheeks. To reduce staining from the antiperspirant, apply it only at night on dry skin. Her blood had coagulated on the front of his fancy jacket, staining it in scarlet bands. If staining is very stubborn, diluted bleach may be used providing the skin is then neutralised with sodium hydrosulphite and rinsed thoroughly. The nerve ring and the nerve bundles that connect the nerve ring and the ventral nerve cord had dots of very faint staining. The sun was tediously ebbing into the horizon, staining the otherwise violet sky with brilliant streaks of orange and vermilion. Four of five strains tested clearly showed staining specifically in the dorsal compartment of the wing disc, as expected for apterous expression. Non-fluorescent techniques for visualizing comets based on staining with silver nitrate have also been used. To retain high-resolution information, chlorosomes were embedded in vitreous ice and imaged without further treatment or staining. Later tissue staining showed that the implants' vessels grew into the host tissue and the host's vessels grew into the constructs. Immunohistochemical staining for cytomegalovirus and herpesvirus was performed to exclude these infections in 4 patients. Use caution to avoid getting overspray on other material due to the risk of staining. My parents gave me a small dog which I love dearly, but she has caused havoc by urinating on the lawn, staining it yellow. Show More Sentences A woman who dared defy this tradition was despised by her family for staining the reputation of her in-laws. Fixation of periderm tissue with FAA did not alter histological staining of cell walls compared with staining of fresh tissues. The structures of rearrangements were defined by mitotic chromosome staining as well as by genetic and polytene chromosome analysis. Small samples were taken from the mixture, and living and dead cells were scored in quadruplicate using trypan-blue staining. When her pail is full she helps me fill my pail, and we both eat handfuls of blueberries, staining our shirts and skirts and skin. Lymph nodes also contain efferent lymphatic vessels, which lack Factor VIII staining. Because our cases were autopsy cases, autolysis may have resulted in a decrease of positive staining cells. For all treatments, plasma membrane cholesterol levels were quantitated by filipin staining as previously described. To stop the palms of your hands from staining, wear latex gloves when you are applying the tanner. If stains are placed afterward, stencils should be left in place until after the staining process is complete. Additional evidence for ongoing denervation of UA muscle in OSA is provided by the significant increase in sarcolemmal N-CAM staining. She bit the sobs back but the tears came anyway, staining her cheeks and the pillow beneath her. The limb staining suggests shared ancestry of the onychophoran and arthropod limbs. Beneath the shallow turf line the loose stoneless silt loam contained slight iron staining and abundant molluscs. In the non-lignified portion, even the highly sensitive aniline blue staining failed to detect the presence of callose, which would be indicative of sieve tube formation. Pollinated stigmas and stylar tissue were softened in some experiments in a solution of 8 N NaOH overnight and washed with fresh water before staining with aniline blue. In the case of plastral staining by tannin and other deposits, the plastron was scrubbed with a stainless steel scourer to allow visibility of the plastral figure. This technique is more reliable than tetrazolium chloride for this species, because some deeply dormant viable seeds show minimal staining with tetrazolium. The man who led the investigation believes the proposals have been watered down so that the staining will not affect the appearance of dog and cat food. First, cytogenetic techniques, especially quinacrine staining, were developed, enabling each chromosome to be recognized by its unique banding pattern. Show More Sentences In addition, numerous plasmacytoid lymphocytes showed strong cytoplasmic staining for IgM and kappa light chains and negative reaction for lambda light chains. One of the oldest antifungal antiseptics available is gentian violet, which is very effective although extremely messy, staining everything it touches. The absence of litter, graffiti and weeds was commended, but the street furniture, bollards, seating and railings would benefit from fresh painting and staining, they said. Micrographs of minerals, metals, pigments, wood sections, and fibers as well as positive and negative microanalytical results for spot tests and fluorescent staining. Use only non-corrosive nails, bolts and screws to prevent staining. We had to hit the shower and put our clothes into the wash immediately to keep them from staining and to keep us from tracking up the whole house with mud and crud. Hall validated her observations by staining the cells with various antibodies that only bind to and illuminate proteins unique to oligodendrocytes precursor cells. It appears as if all of the staining in the cerebral ganglia is due to processes emanating from cells in the ganglia at the base of the tentacles. Congo red staining was resistant to permanganate pretreatment. The distal staining ensheathed the pseudocone and showed tapering extensions toward the basement membrane at ommatidial periodicity, indicative of expression in pigment cells. The second step was a normalization of the staining intensity range to adjust for batch specific background and maximal staining intensity by the chromogen. The small decline in the percentage of cells staining positive for the B cell antigen suggests there may be some modulation of the developing immune system of the foals. Logan glanced at the body and sure enough the throat was slit at the jugular with a trail of blood staining the wood floors, along with a missing ring finger. Wipe up spills in the refrigerator promptly to avoid staining and odors. Leaking pampers and spitting up can soil the material and cause staining. There is also soot staining the tiles, suggesting the bodies were burned or there had been a small blast. Smears of peripheral blood were prepared by conventional manual slide technique and stained by Wright stain or various cytochemical staining methods. Cells were cultured for a further 24 h prior to fixation and staining. Iron staining of the bone marrow aspirate revealed increased storage iron. No acid-fast staining was seen in cases of cryptococcosis or candidiasis. Show More Sentences Healthy mucosa of non-secretors shows little staining for these blood type antigens, but instead, demonstrates significant quantities of the I antigen. As her boyfriend's lifeless body slumps down from the chair onto the floor, spurts of blood splash all over Alice's face and body, staining them red. It poured down his arm and his chest staining the front of his shirt. Until recently, visualization of cytoskeletal filaments was mainly limited to immunofluorescent staining of fixed cells or fluorescent analog cytochemistry for living cells. Eight randomly selected sites from each inflation-fixed right lung were embedded in paraffin, and sections were cut for hematoxylin and eosin staining. Tattooing the skin by pricking and staining with dye reflects the use of woad by Celtic warriors, warpaint by North American Indians, and tattooing by Maoris. Slabs need to be sealed to prevent staining and moss growth. The police, he says, did not help for fear of staining their clothes with the blood. To achieve this, she made the built-in desk and shelves look as much like furniture as possible, using molding at the top of the cabinets and staining the natural wood. Renal biopsy showed severe interstitial nephritis, necrotizing with granulomas and adenovirus staining in the tubular epithelium. Prussian blue staining was performed to quantify intranodular and background liver iron content. Granuloma and staining of the skin from the natural dye inside the sea urchin can also occur. This can result in the precipitation of a certain chemical species producing colouring and staining of the rock, or the formation of concretions. Unstimulated and stimulated cells were fixed and permeabelized to allow intracellular staining of phosphorylated signaling molecules. The response of bacteria to different staining procedures is used in the taxonomic classification of microbes as well. Sequential staining is critical as Pyronin Y can also bind DNA and the prestaining of DNA with Hoechst 33342 blocks this. Many different finishes can be applied to improve the appearance and preserve the surface against staining, water penetration, and freezing. And apoptosis and DNA integrity evaluated by staining the help of the acridine orange. The evaluation of osteogenic or adipogenic properties was then performed through immunocytochemical staining. Further, with our full-section staining we confirmed reactivity within admixed normal adrenal cortex. Show More Sentences The counts of gill rakers and teeth were facilitated by clearing and staining with alizarin red. After 28 days, occurrence of differentiation was examined by alizarin red staining and RT-PCR analysis. Alizarin Red staining was also performed on 2-D hASCs to determine the potential for osteogenic differentiation. Selective Chip Sample MP-016 was taken from an area of argillite and had no visible oxide staining. The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining. The 596 g vertebra has limonitic surface staining and shows significant post-mortem abrasion and degradation. We measured cell cycle distribution and apoptosis using propidium iodide staining and a flow cytometer as described previously by Mandil et al. For the time being, Gram staining and bacterial culture remain the cornerstones of bacterial meningitides diagnosis in a clinical setting. Neither fungal organisms nor malignancy was observed on periodic acid-Schiff and Gomori methenamine silver staining. Callose staining indicates numerous cytoplasmic connections between bundle sheath cell prolongations and neighboring non-vascular cells. Kienast and Schmitz were the first to describe a flow cytometric technique for analyzing retPLTs based on thiazole orange staining. Germicidal mouthwashes such as chlorhexidine do reduce bacteria in the mouth but can also cause staining and an unpleasant taste. Histologic staining indicates these tumors to be positive for periodic acid-Schiff, mucicarmine, and Alcian blue. The staining of the organism capsule with mucicarmine allows differentiation from other fungi. Subsequent staining of the skin sample indicated the presence of spirochete organisms. Today's slide makers and slide stainers are helping laboratories provide fast, cost-efficient, reliable, and uniform staining results. Masson's trichrome staining was also done to assess the collagen formation. Immunohistochemical staining for synaptophysin, neurofilaments, and S-100 protein demonstrated a normal pattern of immunoreactivity. Synaptophysin staining is negative in oligodendrogliomas, although you may see it in the background neuropil. The reference staining method for the manual reticulocyte count uses a dye such as new methylene blue and supravital staining. Show More Sentences Coumassie staining was performed using Bio-Safe Coomassie Stain per the manufacturer's protocol. For each gel, an identical gel was run in parallel and subjected to Coomassie blue staining. Cresyl violet has the advantage of being fluorescent while producing a useful staining pattern like methylene blue with white light. Active cortical remodeling was present, with the osteons filled with new bone centrally as evidenced by the darker histologic staining. This rudimentary vasoformative architecture is clearly demonstrated with reticulin staining. On pathology, papillary cystadenocarcinoma with positive immunohistochemical staining for PSA was confirmed. The properties of MSCs that had differentiated into distinct lineages were evaluated for morphological changes and cytochemical staining. On all media, the placental swab sample yielded moderate growth of tiny colonies, which Gram staining indicated were gram-positive coccobacilli. Microbiology stainers provide high-quality Gram staining and different natural or fluorescent acid-fast stains for mycobacteria. Gram staining was conducted on 3 patients' pleural effusion but none of them showed positive results. Gram staining is one of the most important procedures performed in a microbiology laboratory. Gram staining of a sample revealed many gram-negative diplococci phagocytosed by polymorphonuclear leukocytes. Gram staining revealed Gram-positive diplococci in one case and Gram-positive cocci in another case. If you wait, it may be too late to safely remove the spot without staining or discoloring the fabric. A cytoskeleton will be assessed by immunofluorescent staining with Rhodamine phalloidin. Describe the staining methods for AFB, including fluorochrome and carbolfuchsin stains. Prussian blue staining of broncho-alveolar lavage centrifugate confirmed the presence of hemosiderin laden macrophages. I would start off by using Lithofin Power Clean, which should remove any oily soot and heavy staining. Thyroid transcription factor 1 is a 38-kDa homeodomain protein that shows nuclear-specific staining. Fluorescent staining of specimens provided positive confirmation an abundance of plant roots and fungal hyphae transecting the horn. Show More Sentences The Safranin O staining showed a significantly different proteoglycan profile between the CON group and the injury groups. Several bundles of muscle just above the exocuticle are evident from their strong acidophilia after staining with hematoxylin and eosin. Further examination of the disease course was performed by IF staining of the immunoproteins. To determine the immunoreactive cells, we performed double staining with the immunofluorescence methods. No immunoreactivity was detected when a monoclonal antibody MiTF was used as the primary antibody in the negative control staining. We detected implantation sites by staining the uterus for iron and viewing it under a dissecting microscope. Picro bottles indigo carmine dye for microscopic observation of sperm by staining christmas tree. Expression of glial fibrillary acidic protein has been reported in most cases, although staining is typically focal and of limited intensity. Masson's trichrome staining showed that there were no collagen fibers in the interlobular and portal areas, and few collagen fibers in the central veins and sinusoidal. Silver staining of native and denatured eucaryotic DNA in agarose gels. Histological examination with haemotoxylin and eosin staining was interpreted by the pathologist to be consistent with a 6 mm deep, Clark's level 5 melanoma. Hematoxylin and eosin staining revealed no necrosis or mitotic figures. Negative staining for CD30, TTF-1 and CEA excluded the possibility of embryonal carcinoma and negative TTF-1 staining made a lung or thyroid primary site less likely. Obviously, differences in the method and material used, including the type of hematoxylin, in the staining protocol will affect the staining results. The ratio of the number of vital and dead cells in the suspension was obtained by staining the cells with trypan blue dye and counting in a Neubauer haemacytometer. In addition, phalloidin staining of the actin cytoskeleton and propidium iodide uptake assays were performed to investigate cellular structural integrity. Care should be taken in interpreting Gram staining of isolates because RGM is not identifiable by this method and could be mistaken for corynebacteria or diphtheroids. Methylene blue is an alternative DNA stain that is noncarcinogenic but results in inferior staining and often requires more time for staining and destaining. Once dried, the gels were manually transferred to the staining compartment of the instrument where amido-black staining, destaining, and drying were performed automatically. Before filling into processing cans, the cans were firstly examined for both internal and external defects such as rusting, destaining, staining, pinholes, dents among others. Show More Sentences We describe 2 cases of serogroup C meningococcal disease diagnosed post mortem by PCR from vitreous humor and immunohistochemical staining of tissues collected at autopsy. Use of rapid cytochemical staining to characterize fish blood granulocytes in species of special concern and determine potential for function testing. Immunohistochemical staining for osteocalcin and osteonectin was performed on the confirmed osteosarcoma in the tibiotarsus and the spindle cell sarcoma mass. Fecal analysis by oocyst identification after acid-fast staining or concentration by sugar flotation constitutes a logical first step toward antemortem diagnosis. Cresyl violet staining was used to demonstrate the nissl substance in the neurons and cell nuclei and was used, in this case, to identify neurons. A brilliant cresyl blue staining test can show different intracellular activity of G6PDH in oocytes because the G6PDH enzyme converts BCB from blue to a colorless state. Cajal had imaged synapses by refining Golgi's staining technique. The sections from lowest PMI and longest PMI were immunostained with antibodies to GFAP and neurofilament to note if there is any difference in staining character. With regard to elucidating the antithrombogenicity of the endothelial cells, we performed additional staining with anti-thrombomodulin and anti-tissue factor. Cytoplasmic borders are difficult to identify, and special staining with Alcian blue dye will highlight the mucopolysaccharides abundant in this tumor type. Special stains, including Fite staining for acid-fast bacteria and Gomori methenamine silver staining were performed, and no microorganisms were recognized. In contrast, the expression host itself, a DE3 lysogen induced with IPTG but harboring no expression plasmid construct, exhibited fairly uniform staining and morphology. To evaluate the biometry, thin section slides for light microscopy were prepared following the usual methods of softening, cutting, staining, and mounting. A combination of cell size, myeloperoxidase staining and nuclear lobulation was used to perform the total leucocyte and 6-part differential count. While there is some debate over whether rhabdomyosarcomas indeed arise from skeletal muscle cells, the staining pattern is consistent with that differentiation. Calcification will be visually observed using alizarin staining. Biological characteristics of the cells were then examined using MTT assays, AKP activity assays, Alizarin Red S staining, and western-blotting, respectively. Even though no obvious staining was observed in our case, contact with the vapor was still found to perform very faint etching at a submicrometric scale. Defects in chromatin assembly and the nucleolus were also confirmed histologically where abnormal patterns of chromatin staining and nucleomegaly were observed. Note the presence of intracytoplasm and perinuclear staining. Examples from Classical Literature The staining was not difficult work but the nails made it splashy and we were pretty well spotted when we finished. This staining can only be limited by dilution, but not by the addition of opposed dyes. Some workers as noted above have been able to demonstrate collections of nuclein by staining, especially in very young cells. The fault can be remedied by staining the line to match the surface of the leather. There was another, crushed and faded, and staining the leaves with its purple blood. Traces of inflammation and discoloration by sanguineous staining are traceable on the pleural surfaces. The staining of the blood specimen in triacid requires only a little fixation, cp. The nucleus soon enlarges and a large dense body appears which stains like chromatin with various staining media. With faint staining, a thin peripheral layer of the eosinophil granule is seen more deeply stained than the interior. This resembles that for the tubercle bacillus, viz., drying on a cover glass and staining with fuchsin or methyl-olin. There is much danger of staining the conscience in affairs like these. A ghost of color was going up her throat, staining her cheeks. It gushed down his chin, staining the cleanness of his robe. Water staining in the loft is an early warning sign of a problem with the roof covering, so get it looked at by a reputable roofer straightaway. The directions for staining will be found on the can in which it is sold. What should we have in mind when staining furniture for the home? That trick of staining the fishes' scales of a delicate pink is quite peculiar to China. The interrelationship of bark beetles and blue staining fungi in felled Norway pine timber. Single staining is attained on the principle of maximal decoloration. It'll catch the light on the tops of your shoulders, collarbones and decollete without staining your clothes. Show More Sentences It involves snipping a tiny piece of lymphoid tissue from the animal's nictitating membrane, or third eyelid, staining it with antibodies, and examining it under a microscope. Histologically, lymph node aspirates are characterized by the presence of small, pleomorphic, gram-negative, argyrophilic bacilli on Warthin-Starry staining. The room was empty save for herself and the still form of the jeddak of Lothar lying at her feet, a little pool of crimson staining the white marble of the floor beside him. Find more words! 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What is the opposite of staining? Words that rhyme with staining What is the adverb for staining? What is the adjective for staining? What is the noun for staining? Use our Sentence Generator Nearby Words stainer stained glass stained stainable Stain stain stainless stainlessness stainless-steel stains stair Stair 8-letter Words Starting With s st sta stai stain staini stainin